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BP: Fachverband Biologische Physik
BP 10: Poster III
BP 10.1: Poster
Montag, 16. März 2020, 17:30–19:30, P2/3OG
A time resolved study of blood platelet spreading — •Anna Zelena1, Magdalena Haaf1, Sebastian Isbaner2, Daja Ruhlandt2, Anna Chizhik2, Alexey Chizhik2, Jörg Enderlein2, Ulrich S. Schwarz3, and Sarah Köster1 — 1Institute for X-Ray Physics, University of Göttingen, Germany — 2Third Institute of Physics - Biophysics, University of Göttingen, Germany — 3Institute for Theoretical Physics, Heidelberg University, Germany
Human blood platelets are non-nucleated fragments of larger cells (megacaryocytes) and of high importance for blood clotting. The hemostatic function of platelets is directly linked to their mechanics and cytoskeletal morphology. However, the exact mechanism of spreading and contraction remains elusive. In our study we focus on the investigation of single blood platelets in vitro employing Traction Force Microscopy (TFM) and Metal-Induced Energy Transfer (MIET) imaging. By combined TFM and microscopy, we are able to correlate the force generation with the emerging actin structures in a time resolved manner. Our force maps show a hot spot distribution, typically in spindle-like, triangular or circular shape. Additionally, from fast scanning and static MIET experiments, we reconstruct the temporal evolution of the membrane-to-surface distance during adhesion and spreading with nanometer resolution. We observe in MIET three-dimensional height profiles, analogous to the TFM, hot spot distribution shapes of areas with lower membrane-to-surface distances.