Dresden 2020 – scientific programme

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BP: Fachverband Biologische Physik

BP 10: Poster III

BP 10.11: Poster

Monday, March 16, 2020, 17:30–19:30, P2/3OG

Determining the viscoelastic shear modulus of zebrafish embyros during embryogenesis on the tissue scale — •Julian Vondereck, Sebastian Hurst, Bart Vos, and Timo Betz — University of Münster, ZMBE, Institut of Cell Biology

Understanding the physical principles of collective cell movements is key to acquiring deeper knowledge about fundamental biological processes ranging from wound healing over metastatic cancer invasion to development. A distinguished model to study these processes is collective cell migration in embryogenesis. The first coordinated tissue motion in a zebrafish embryo is called epiboly, where the blastocyte cells spreads over the yolk cell. To understand the mechanical properties during this process, we use in vivo, optical tweezer based microrheology. Epiboly can be described by the behavior of the three embryonic regions: the epithelial monolayer or enveloping layer (EVL), the yolk syncytial layer (YSL) and the deep cells (DEL) of the blastoderm. EVL, YSL and DEL all undergo epiboly. 10 micrometer sized particles are microinjected in these regions as tissue-based probes. Microrheology in vivo allows to determine the viscoelastic shear modulus on the tissue scale, while resolving spatial and temporal changes during development. Illuminating cell and tissue differentiation on the different regions in general and at certain key points is to be expected. This provides new insights into the biomechanical processes controlling collective cell movements, of which a detailed understanding yet remains elusive.