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BP: Fachverband Biologische Physik
BP 10: Poster III
BP 10.18: Poster
Montag, 16. März 2020, 17:30–19:30, P2/3OG
Characterization of cell deformability in patients with major depressive disorder — •Lisa Kwapich1, Tobias Neckernuss1,2, Daniel Geiger1,2, Jonas Pfeil1,2, Patricia Schwilling1, Alexander Karabatsiakis3, Iris-Tatjana Kolassa3, and Othmar Marti1 — 1Institute of Experimental Physics, Ulm University — 2Sensific GmbH — 3Institute of Psychology and Education, Ulm University
Major depressive disorder is a debilitating disease that affects more than 300 million people worldwide. Despite advances in the understanding of the etiology of major depression, no established mechanism can explain all aspects of the disease. A promising approach in this context is the analysis of mechanical cell properties by deformability cytometry. The method probes cell stiffness at high throughput by exposing cells to a shear flow in a microfluidic channel, allowing for mechanical phenotyping based on single-cell deformability. The advantage of this method is that cells are purely deformed by hydrodynamic interactions and without contact with channel walls. The deformability of cells is indicative of underlying membrane, cytoskeletal, or nuclear changes associated with changes in cell state and various disease processes. We investigated differences in deformability of peripheral blood mononuclear cells between patients with major depressive disorder and non-depressed control subjects. A custom-built algorithm (ODIN technology) was used for image analysis, data collection, and postprocessing analysis. The algorithm detects the presence of a cell, determines its contour, and quantifies several parameters.