Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 10: Poster III
BP 10.20: Poster
Montag, 16. März 2020, 17:30–19:30, P2/3OG
Adherent Cell Optical Stretcher: Quantifying Laser Induced Heating — •Alexander Janik1, Tobias Neckernuss1, Cornelius Maurer1, Seiichi Uchiyama2, and Othmar Marti1 — 1Institute of Experimental Physics, Ulm University — 2Graduate School of Pharmaceutical Sciences, The University of Tokyo
Cell stiffness is a valuable indicator for cell functionality, especially in cell types that naturally undergo strong mechanical deformation, such as alveolar epithelial cells. We therefore recently developed a method to stretch adherent cells with a parallel laser beam to determine their viscoelastic properties.
The impact on cells is kept low, for they are stretched uniformly, and the procedure does not require fluorescent labeling. However, heating induced by the 800 nm stretching laser is a side effect. Our contribution focuses on quantification and real-time monitoring of intracellular temperature change during the stretching process. A ratiometric temperature dependent fluorescent dye is used whose two emission bands are detected simultaneously on two cameras. Improvements of the setup for cell height detection, which currently employs beads as markers, are also discussed.