Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster V
BP 17.20: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/1OG
Physical properties of cells required for efficient microcirculation — •Martin Krater1,2, Stefanie Tietze2, Angela Jacobi1,2,3, Anna Taubenberger2, Martin Bornhauser3, and Jochen Guck1,2 — 1MPI for the Science of Light Erlangen — 2Biotechnology Center TU Dresden — 3Medical Clinic I, University Hospital TU Dresden
Biological cells in blood encounter successive constrictions smaller than their own diameter when circulating through microcapillaries such as the pulmonary networks. This is relevant for blood cells, circulating tumor cells or cells transplanted for therapeutic purposes such as mesenchymal stromal cells (MSC). While the cell size has been shown to be a relevant parameter to overcome capillary entrapment, the cells mechanical properties have so far not been considered. Here we investigated the microcirculation of MSCs as a function of their physical phenotype quantified using real-time deformability cytometry and atomic force microscopy. When MSCs were expanded in organ-like 3D mesenspheres, as opposed to MSCs classically cultured on 2D surfaces, we found them to be smaller and more compliant. Resulting in a more effective circulation in vitro, using a microfluidic microcirculation mimetic and improved in vivo circulation after intravenous transplantation to NOD/SCID mice. The initially large and stiff MSCs cultured in 2D could be reprogrammed into a physical phenotype suitable for circulation by subsequent culture in 3D systems. Thus, the physical properties of cells are essential to overcome capillary entrapment and are a promising therapeutic target to improve effective circulation.