Dresden 2020 – wissenschaftliches Programm
Die DPG-Frühjahrstagung in Dresden musste abgesagt werden! Lesen Sie mehr ...
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 17: Poster V
BP 17.22: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/1OG
Mechanical dissociation of tissue for real-time deformability cytometry — •Markéta Kubánková1, Despina Soteriou1, Oana-Maria Thoma2, Andrea-Hermina Györfi3, Alexandru-Emil Matei3, Stefan Scheuermann4, Felix Dirla5, Maximilian Waldner2, Jörg Distler3, Jens Langejürgen4, and Jochen Guck1 — 1Max Planck Institute for the Science of Light, Erlangen, DE — 2Dept. of Medicine 1, FAU Erlangen-Nürnberg, DE — 3Dept. of Medicine 3, FAU Erlangen-Nürnberg, DE — 4Fraunhofer IPA, Mannheim, DE — 5BioITix, Frankfurt/Main, DE
Real-time deformability cytometry (RT-DC) is a high-throughput method used for characterizing single cells in suspension. Here we apply RT-DC for analyzing cells obtained from solid tissues. We used an enzyme-free approach based on mechanical dissociation of tissue, TissueGrinder (TG), to obtain heterogeneous single cell suspensions from various mouse tissues. The dissociation procedure resulted in high cell yield and viability and was significantly faster (~ 20 min) compared to conventional enzymatic methods (hours). RT-DC analysis allowed us to distinguish subpopulations of cells in label-free manner and to extract further information from single cell images, such as deformability. A key advantage of this method is that it is non-destructive and cells may be further reused. Moreover, a cell population of interest can be sorted according to image-based parameters. The combination of TG with RT-DC has potential as a fast and high-throughput diagnostic pipeline to detect pathological changes in tissue biopsies and to reveal cell populations invisible to marker-based approaches.