Dresden 2020 – scientific programme
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BP: Fachverband Biologische Physik
BP 17: Poster V
BP 17.28: Poster
Tuesday, March 17, 2020, 14:00–16:00, P2/1OG
Dynamic RT-DC: red blood cell viscoelasticity as a label-free biomarker — •Bob Fregin1,3, Fabian Czerwinski1, Konstanze Aurich2, Doreen Biedenweg2, Stefan Groß3, Gerlad Kerth4, and Oliver Otto1,3 — 1ZIK HIKE, Universität Greifswald, Germany — 2Universitätsklinikum Greifswald, Germany — 3DZHK, Universität Greifswald, Germany — 4Angewandte Zoologie und Naturschutz, Universität Greifswald, Germany
Real-Time Deformability Cytometry (RT-DC) is a label-free technique for single cell mechanical analysis with high-throughput of up to 1,000 cells/second. Initially, RT-DC was limited to steady-state deformation captured at the end of the channel enabling the calculation of time-independent information as the Young's modulus.
We introduce an extension of RT-DC towards dynamic single cell measurements with the possibility to capture full viscoelastic properties at up to 100 cells/s. Cellular shape-changes along the entire length of the microfluidic channel are tracked in real-time and are subsequently analyzed by a Fourier decomposition discriminating cell responses to interfering stress distributions. We demonstrate that dRT-DC allows for cell mechanical assays at the millisecond time scale independent of cell shape. We use this approach for a comparison of peripheral blood cells based on their Young's modulus and viscosity.
In proof-of-principle experiments we use dRT-DC to approach the question of temperature control in hibernating animals. Initial experiments on bats and humans suggest a role of red blood cell viscoelasticity to maintain blood flow at low temperatures.