Dresden 2020 – scientific programme

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BP: Fachverband Biologische Physik

BP 17: Poster V

BP 17.29: Poster

Tuesday, March 17, 2020, 14:00–16:00, P2/1OG

Stimulation Dynamics of Circular Dorsal Ruffles in Fibroblast Cells — •Malte Ohmstede and Hans-Günther Döbereiner — Institut für Biophysik, Universität Bremen, Bremen

Circular Dorsal Ruffles (CDRs) are ring shaped, propagating protrusions on the dorsal cell surface in - among others - fibroblast cells. These dense actin structures play a major role in macropincytosis and are orchestrated using a complex molecular pathway. The upstream stimulation of CDRs is done by activation of Receptor Tyrosine Kinases using various types of growth factors. In this work, Platelet Derived Growth Factor (PDGF) in conjunction with a microfluidic perfusion chamber is used to perform precise stimulation and re-stimulation of adherent fibroblast cells. Combined with fibronectin micro-contact printing, this method reproducibly yields large amounts of data from equally shaped cells. Through usage of circular kymographs and further image processing, the dynamics of cellular responses to PDGF stimuli are measured. For different PDGF concentrations, various characteristics of CDRs are evaluated. CDRs only form on the lamellipodium of adherent cells and are reflected by the nucleus and the cell edge. Incorporating deep learning based image segmentation, the influence of lammelipodial shape as boundary condition on CDR dynamics is evaluated.