Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster V
BP 17.2: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/1OG
Tailoring Network Structure of Collagen Type 1 by High-Energy Electron Irradiation — •Catharina Krömmelbein1, Stefanie Riedel1, Tom Kunschmann2, and Stefan Mayr1,2 — 1Leibniz-Institut für Oberflächenmodifizierung e. V., Leipzig, Germany — 2Fakultät für Physik und Geowissenschaften, Universität Leipzig, Leipzig, Germany
Imitating mammalian’s extracellular matrix is essential to investigate cell behavior like adhesion and migration in vitro and is of great importance for biomedical applications as well. Collagen as main component of the mammalian’s extracellular matrix is well suited as biomimetic material due to its excellent biocompatibility and biodegradability. Mostly, the fabrication of collagen type 1 matrices with defined network pore size involves the usage of cytotoxic chemicals. Herein, we adjust the pore size by covalent crosslinking, using high-energy electron irradiation of 0 kGy, 50 kGy and 100 kGy. Thereby, electron beam treatment is an effective and noninvasive method to ensure biocompatibility without the need for further chemicals. In addition, the pore size dependence of collagen type 1 networks on protein concentration is examined, using 1 mg/ml, 2 mg/ml and 3 mg/ml. In this contribution, we present a method to tailor the hydrogel’s pore size by adjusting the gel concentration and the irradiation dose.