Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 19: Poster VII
BP 19.20: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/3OG
Probing Nucleosome Dynamics in Magnetic Tweezers — •Yi-Yun Lin1, Willem Vanderlinden1, Lori Van de Cauter1, Tine Brouns1,2, and Jan Lipfert1 — 1Department of Physics, Nanosystems Initiative Munich, Center for NanoScience, LMU Munich, Amalienstrasse 54, 80799, Munich, Germany — 2KU Leuven, Division of Molecular Imaging and Photonics, Celestijnenlaan 200F, 3001, Leuven, Belgium
Nucleosomes are the fundamental unit of chromatin. They control eukaryotic genome accessibility and can regulate expression, replication and repair of the genome by organizing chromatin. Multiple factors affect chromatin dynamics and control the unwrapping and assembly of nucleosomes. Lens epithelium-derived growth factor (LEDGF) p75 is a co-activator of general transcription. In vivo LEDGF/p75 can recognize transcriptionally active genomic regions by specific binding of its PWWP domain to tri-methylated histone H3 lysine 36 (H3K36me3). To investigate how LEDGF/p75 affects chromatin structure, we applied single molecule magnetic tweezers. Our results suggest that LEDGF/p75 alters nucleosome unwrapping and inter-nucleosome interactions, and help us understand the cellular mechanisms of LEDGF/p75 as a transcriptional co-activator.