Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 19: Poster VII
BP 19.22: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/3OG
Avidity of multivalent DNA binding to DNA origami — •Maximilian Voggenthaler1, Ricarda Berger1, Joachim Rädler1, Ralf Jungmann2, Florian Schüder2, Alexander Auer2, Eugene Petrov1, and Tim Liedl1 — 1Department of Physics and Center for Nanoscience, Ludwig Maximillian University, Munich, Germany — 2Max Planck Institute of Biochemistry, Martinsried, Germany
Multivalent binding is a frequently occurring theme in biological recognition that enables both tight binding and high specificity. DNA nanotechnology employs this concept when higher order assemblies are designed by multivalent hybridization sites. However, the effective binding affinity of multiple linkages with both steric as well as electrostatic constraints has not been studied in a quantitative manner. Here, we study the binding affinity of a double stranded DNA helix with two single stranded overhangs to a DNA origami block equipped with two complementary strands as multivalent receptor. We use fluorescence correlation spectroscopy, thermophoresis, total internal reflection fluorescence microscopy and gel electrophoresis to study avidity as a function of spacing and salt concentration. Our findings are important for better engineering of super selective linkages in origami self-assembly and medical nanoagents.