Dresden 2020 – scientific programme

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BP: Fachverband Biologische Physik

BP 19: Poster VII

BP 19.24: Poster

Tuesday, March 17, 2020, 14:00–16:00, P2/3OG

Optical tweezers and multimodality imaging: a platform for dynamic single-molecule analysis — •Ann Mukhortava, Baerbel Lorenz, Philipp Rauch, and Andrea Candelli — LUMICKS B.V. Amsterdam, Pilotenstraat 51, 1059CH Amsterdam, The Netherlands

The possibility to investigate molecular interactions, structure, and dynamics using single-molecule fluorescence- and force spectroscopy-based methods has led to many new insights over the past decades. Here, we present our efforts in establishing the easy and reliable experimental workflow for further enabling discoveries in the field of biology and biophysics using both the combination of optical tweezers with single-molecule fluorescence microscopy (C-Trap). As a proof of concept, we will discuss an overview of the experimental designs and the workflow for combining FRET with an ultra-stable optical trap for studying binding and colocalization dynamics of histones and a helper protein on DNA and observing protein/DNA hairpin folding dynamics. These experiments show that the technological advances in hybrid single-molecule methods can be turned into an easy-to-use and stable instrument that opens up new venues in many research areas.