Dresden 2020 – scientific programme

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BP: Fachverband Biologische Physik

BP 20: Poster VIII

BP 20.10: Poster

Tuesday, March 17, 2020, 14:00–16:00, P2/4OG

Micropatterning on Gold Surfaces for Biophysical Applications — •Carolin Grandy, Peter Kolb, Fabian Port, and Kay-E. Gottschalk — Institute of Experimental Physics, Ulm University, Ulm, Germany

Cells change their shape based on their environment and are highly sensitive to mechanical and geometric factors. Micropatterning is a simple method to manipulate and control cell shape [1]. Our primary goal was to develop a reproducible method to manipulate and normalize cell shape on gold with micropatterning for quantitative super-resolution imaging. We use methoxy polyethylene glycol (PEG) thiol to create a self-assembled monolayer on gold, due to the strong bonds formed between thiol groups and gold [2], and the protein repellent nature of PEG. This monolayer can be oxidised through a photomask with deep UV-light [1]. The oxidised PEG pattern is no longer protein repellent, and can be coated with extracellular matrix proteins and seeded with cells. The surfaces we developed and analyzed can be used in a wide range of biophysical applications, including metal induced energy transfer and surface enhanced raman scattering.

References:

[1] M Thery, Micropatterning as a tool to decipher cell morphogenesis and functions, Journal of Cell Science 2010 123 (24), 4201-4213.

[2] R.G. Nuzzo et al, Fundamental studies of the chemisorption of organosulfur compounds on gold(111). Implications for Molecular self-assembly on gold surfaces, Journal of the American Chemical Society 1987 109 (3), 733-740.