Dresden 2020 – scientific programme

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BP: Fachverband Biologische Physik

BP 20: Poster VIII

BP 20.15: Poster

Tuesday, March 17, 2020, 14:00–16:00, P2/4OG

Development of a fast real time sub-pixel accurate multi particle tracking system — •Jonas Pfeil, Daniel Geiger, Tobias Neckernuss, and Othmar Marti — Institute of Experimental Physics, Ulm University, Ulm, Germany

A multitude of functions in the human body depend on the rheological behaviour of the tissue and hence of the single cells involved. To determine the microscopical cell properties there is a need for microrheological measurement devices. An established technique is the passive and active microrheology. Small beads with known properties embedded in the probed material are tracked by an imaging system. By comparison with theoretical models, mechanical properties of the cells can be computed.

We present a new implementation of a system for particle tracking which can track up to 8 particles with sub pixel precision at high speeds of up to 10,000 frames per second. We will discuss the setup, the implemented method for subpixel tracking and the hardware consisting of an FPGA and a CMOS sensor.

In addition we will show a fast and fixed latency algorithms for the approximative multi-parameter fitting of 2D surfaces.