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BP: Fachverband Biologische Physik
BP 20: Poster VIII
BP 20.17: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/4OG
High throughput sorting in droplet-based microfluidics — •Patricia Schwilling1, Tobias Neckernuss1,2, Daniel Geiger1,2, Jonas Pfeil1,2, Ralf Schuster1, Lisa Kwapich1, and Othmar Marti1 — 1Institute of Experimental Physics, Ulm University — 2Sensific GmbH
Droplet-based microfluidics offer a method to meet the ever-increasing demand for cost-efficient high-throughput analysis and experiments by fabricating up to thousands of nanoliter-sized water-in-oil droplets per second. Each droplet serves as a tiny reaction chamber in which for example specific biochemical reactions may take place. One field, among others, that utilizes this technique is single-cell RNA sequencing by encapsulating functionalized beads with cells to capture the cells’ RNA for subsequent sequence analysis. It is therefore inevitable to encapsulate single cells with single beads to ensure the highest resolution. Here, we present a setup to eliminate droplets containing more than one cell together with more than one bead using dielectrophoretic force and ODIN - a high-performance optical sensing system for real-time counting, analysis, and sorting. The setup is further capable to sort biological and synthetic particles and complex structures in a continuous flow based on their respective mechanical or phenotypical properties.