Dresden 2020 – scientific programme
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BP: Fachverband Biologische Physik
BP 20: Poster VIII
BP 20.26: Poster
Tuesday, March 17, 2020, 14:00–16:00, P2/4OG
Low-budget High-resolution Fluorescence Microscope — •Leon Claassen1, Lukas Lechler1, Florian Schwarz1, and Jens Pflaum1,2 — 1Experimental Physics VI, Julius Maximilian University of Würzburg, 97074 Würzburg — 2ZAE Bayern, Würzburg
Non-destructive optical methods are vital for the determination of material properties in various fields of application. In particular, fluorescence microscopy has become an established technique which can be used for analyzing solid state properties such as the optical band-gap of semiconductors or in biology to identify and spatially resolve labeled constituents in cells or cellular processes. However, fluorescent microscope setups are usually very expensive and thus, financially hardly viable for schools or technical classes. Here, we present a low-cost laser-scanning version of such a setup based on two DVD-scanners driven by an Arduino controller and slightly modified optics, which offers a resolution up to 15 µ m within a scanning range of 5 x 5 cm2. We demonstrate the characteristics of the relevant electronic and optical components and show first results obtained by operating the microscope in a neat reflection mode. Thereafter, the necessary conversions to run the setup in fluorescent mode are presented together with preliminary studies on fluorescent organic single crystals as well as labeled biological structures. Based on these achievements we will discuss future optimizations to improve the performance and, in particular, the lateral resolution of the setup down to the micrometer length scale. The University of Würzburg is acknowledged for financial support within the Fund for Innovative Projects in Teaching.