Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 20: Poster VIII
BP 20.6: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/4OG
Biocompatible fluorescence modulating nanocoatings for sharper and faster screening applications — •Deyan Nestorov1, Julia Fender2, Kristina Lorenz2, and Katrin G. Heinze1 — 1Rudolf Virchow Center, University of Würzburg, 97080 Würzburg, Germany — 2Institute of Pharmacology and Toxicology, University of Würzburg, 97078 Würzburg, Germany
Biocompatible metal-dielectric nanocoatings have been shown to act as versatile fluorescence modulators. They can be customized to different spectral ranges and biological architectures of interest. Here, we explore such coatings for dual-color high-throughput screening (HTS) applications highlighting ligand-receptor interactions. Culturing adherent cells directly on nanocoated substrates allows to enhance both the signal-to-noise ratio of the detected fluorescence from the fluorescenctly tagged membrane proteins as well as the Förster resonance energy transfer (FRET) efficiency as a measure of the protein conformational changes upon ligand binding. Our simulations show that the signal contrast could be enhanced by a factor of 2.7, and thus the screening rate accelerated by a factor of 1.6. We plan to experimentally establish such an approach for high-throughput ligand screening of G-Protein coupled receptors which comprise over 30% of all drug targets. A major advantage of this approach is that it could be directly integrated into common setups with ease and thus offers new perspectives for pharmacological research.