Dresden 2020 – scientific programme

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BP: Fachverband Biologische Physik

BP 20: Poster VIII

BP 20.7: Poster

Tuesday, March 17, 2020, 14:00–16:00, P2/4OG

Metal Induced Energy Transfer for Structural Analysis of Focal Adhesions via Actin Stress Fiber Angles — •Lydia Rebehn, Fabian Port, and Kay-E. Gottschalk — Institute of Experimental Physics, Ulm University, Ulm, Germany

Focal adhesion (FA) structures physically anchor cells to the extracellular matrix and facilitate cell interactions with their environment. They are composed of different protein components assembled in a highly regulated conformation. Intracellularly, FAs couple with actin to transfer external forces into internal mechanical and biochemical signals. Their structure is difficult to resolve since the components interact in the nanometer range [1],requiring localization of molecular components with nanometer-range resolution in x-, y-, and z- axes. Analyzing the actin stress fibers of cells in 2D culture can give information on the FA attachment to the surface based on angle analysis. The technique we use is Metal Induced Energy Transfer (MIET) [2]. We have provided an initial analysis of the angles between actin stress fibers and a 2D substrate, demonstrating the worth of MIET for cellular structure analyses near the basal membrane with nanometer accuracy.

References:

[1] Kanchanawong, P., Shtengel, G., Pasapera, A. M., Ramko, E. B., Davidson, M. W., Hess, H. F., & Waterman, C. M.,Nature, 468(7323), 580-584 (2010)

[2] Chizhik, A. I., Rother, J., Gregor, I., Janshoff, A., Enderlein, J., Nature Photonics, advance on(January), 1-8 (2014)