Dresden 2020 – wissenschaftliches Programm

Die DPG-Frühjahrstagung in Dresden musste abgesagt werden! Lesen Sie mehr ...

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 20: Poster VIII

BP 20.9: Poster

Dienstag, 17. März 2020, 14:00–16:00, P2/4OG

Investigation of Actin near the Basal Membrane of Living Cells using Metal Induced Energy Transfer — •Fabian Port, Ulla Nolte, Peter Kolb, Carolin Grandy, and Kay-E. Gottschalk — Institute of Experimental Physics, Ulm University, Germany

Focal adhesions function as anchoring points to the extracellular matrix, and also enable cells to sense and exert forces on their environment [1]. Focal adhesions are complex structures consisting of a multitude of different proteins. Despite the important role of the focal adhesion complex in cellular adhesion, its structure and dynamics remain difficult to resolve [2]. Knowing the exact position of the proteins in the focal adhesion complex in live cells is necessary to understand their working principles. For a detailed analysis of the focal adhesions dynamic architecture, we require a method to measure small distances that may be applied over a variable time scale. To meet this challenge, we use Metal Induced Energy Transfer (MIET) [3] to resolve protein positions at the nanoscale level in live cells. Using MIET, we analyse the dynamics of focal adhesion associated actin with ultra high resolution.

[1] Geiger, B. et al., Nature Reviews. Molecular Cell Biology, 10(1), 21-33 (2009)

[2] Kanchanawong, P. et al., Nature, 468(7323), 580-584 (2010)

[3] Chizhik, A. I. et al., & Enderlein, J., Nature photonics, advance on(January), 1-8 (2014)