Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 23: Focus: Physics of Stem Cells
BP 23.1: Vortrag
Mittwoch, 18. März 2020, 09:30–09:45, ZEU 250
Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish — Gopi Shah1, Konstantin Thierbach2, Benjamin Schmid1, Johannes Waschke3, Anna Reade4, Mario Hlawitschka3, Ingo Roeder2, •Nico Scherf1,2, and Jan Huisken1 — 1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. — 2Institute for Medical Informatics and Biometry, TU Dresden, Germany — 3Faculty of Computer Science and Media, Leipzig University of Applied Sciences, Germany — 4Department of Biochemistry and Biophysics, University of California San Francisco, USA
The coordination of cell movements across spatio-temporal scales ensures precise posi- tioning of organs during vertebrate gastrulation. Mechanisms governing such morphogenetic movements have been studied only within a local region, a single germlayer or in whole embryos without cell identity. Scale-bridging imaging and automated analysis of cell dynamics are needed for a deeper understanding of tissue formation during gastrulation. Here, we report pan-embryo analyses of formation and dynamics of all three germlayers simultaneously within a developing zebrafish embryo. We show that a distinct distribution of cells in each germlayer is established during early gastrulation via cell movement char- acteristics predominantly determined by position within the embryo. The differences in initial germlayer distributions are amplified by a global movement, organizing the organ precursors along the embryonic body axis and giving rise to the blueprint of organ formation.