Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 30: Cell Adhesion and Migration, Multicellular Systemadhesion and Migration, Multicellular Systems II
BP 30.11: Vortrag
Donnerstag, 19. März 2020, 12:45–13:00, HÜL 386
Nanoprobing of osteoblasts adhered to micro-contact printed dendrimer and protein layers — •Christian Völkner1, Issam Assi1, Martina Grüning2, Regina Lange1, Barbara Nebe2, Ingo Barke1, and Sylvia Speller1 — 1Institute of Physics, Physics of Surfaces & Interfaces, University of Rostock, 18059 Rostock — 2University Medical Center, Dept. of Cell Biology, University of Rostock, 18057 Rostock
Chemical and physical surface gradients to control local cell adhesion and migration may allow to find routes to improve osseointegration of implants. Therefore, the local as well as the mesoscopic responses of living osteoblast-like cells (MG-63) were studied by means of Scanning Ion Conductance Microscopy (SICM) [1] and Fluorescence Microscopy, respectively. To achieve molecular landscapes with a small topographic corrugation height, amine-terminated PAMAM dendrimers and albumin were deposited in a stripe pattern on glass cover slips by direct micro-contact printing [2]. A distinct spindle shape oriented parallel to the surface pattern as well as a preferential adhesion of the cells on the glass site is observed when the width of the stripes is in the regime of 20 microns. We discuss in how far the pre-treatment of the glass and small protruding heights of a few nm are involved in the preference of the cells.
[1] Korchev et al., Biophys. J. 73, 653 (1997)
[2] Whitesides et al., Chem. Rev. 105, 1171 (2005)