Dresden 2020 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 30: Cell Adhesion and Migration, Multicellular Systemadhesion and Migration, Multicellular Systems II

BP 30.9: Vortrag

Donnerstag, 19. März 2020, 12:15–12:30, HÜL 386

Cell Motility Using Race Tracks on Elastic Substrates — Daniel Meyer¹, Christoph Schreiber², Joachim Rädler², Matthias Weiss³, and •Florian Rehfeldt^1,3 — ¹3rd Institute of Physics - Biophysics, Georg-August University, Göttingen, Germany — ²Faculty of Physics, Soft Condensed Matter Group, Ludwig-Maximilians-University, Munich, Germany — ³Experimental Physics 1, University of Bayreuth, Bayreuth, Germany

Cell motility and migration processes are vital during biological development and homeostasis. They are essential in tissue regeneration, morphogenesis, but also in pathological mechanisms like tumor metastasis. While migration due to biochemical gradients (e.g. chemotaxis) is very well studied, the influence of other parameters of the micro-environment such as topography and stiffness are less understood.

Here, we use polyacrylamide (PA) hydrogels in combination with a novel microcontact printing (µCP) protocol to generate patterned substrates with well-controlled Young’s modulus E . These collagen-coated tracks are used to analyze the migration behavior of human mesenchymal stem cells (hMSC) and NIH3T3 fibroblasts by parallelized life cell microscopy under physiological conditions.

We demonstrate that both, elasticity as well as the geometry of the tracks affect the migration velocity and that the two cell types show distinct motile behavior.