Dresden 2020 – scientific programme
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BP: Fachverband Biologische Physik
BP 33: Protein Structure and Dynamics
BP 33.1: Talk
Thursday, March 19, 2020, 09:30–09:45, ZEU 250
Protein Short-Time Diffusion in a Naturally Crowded Environment — Marco Grimaldo1, Hender Lopez1,2,3, •Christian Beck1,2, Olga Matsarskaia1, Felix Roosen-Runge5, Martine Moulin1, Juliette Devos1, Valerie Laux1, Michael Hartlein1, Stefano Da Vela2, Ralf Schweins1, Alessandro Mariani4, Fajun Zhang2, Jean-Louis Barrat3, Martin Oettel2, V. Trevor Forsyth1,6, Tilo Seydel1, and Frank Schreiber2 — 1Institut Laue-Langevin, Grenoble, France — 2University of Tübingen, Germany — 3LiPhy, Saint Martin d’Hères, France — 4European Synchrotron Radiation Facility, Grenoble, France — 5Malmö University, Malmö, Sweden — 6Keele University, Staffordshire, UK
We employ neutron backscattering spectroscopy to measure the short-time self-diffusion of tracer proteins in a deuterated cell-like environment (cell lysate) with explicit control over crowding conditions. We successfully link coarse-grained Stokesian dynamics simulations with experimental results on these complex, flexible molecules providing a consistent understanding by colloid theories. In the case of immunoglobulin, both experiments and simulations show that tracers in polydisperse solutions close to the effective particle radius Reff=⟨ Ri3⟩1/3 diffuse approximately as if the suspension was monodisperse [1]. The simulations predict size-dependent deviations from this scaling which was tested by measuring different proteins in lysate with an increased energy transfer range, also allowing to investigate the influence of the lysate on the internal dynamics in more detail. [1] M. Grimaldo et al.; J. Phys. Chem. Lett. 10 (2019) 1709