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BP: Fachverband Biologische Physik

BP 37: Systems Biology, Evolution and Neural Networks I

BP 37.3: Vortrag

Donnerstag, 19. März 2020, 15:45–16:00, ZEU 250

Sequence selection of oligonucleotides under a ligation chain reaction — •Patrick Kudella and Dieter Braun — Systems Biophysics, Ludwig-Maximilians-Universität München

Replication of information on oligonucleotides such as RNA or DNA is essential for the emergence of life. Previous studies focus on the replication of single sequences, but we believe it is the key to monitor selection dynamics and replication starting in an already completely random pool of sequences.

We expect a nonlinear ligation dynamic had set in, once polymerization was able to create oligomers long enough for hybridization and thus capable of structure formation and templated ligation. We study if certain sequences could have been selected at this onset of replication that potentially lead to interesting non-linear and frequency-dependent behavior.

By using adenine-thymine-only 12mers long random-sequence strands as starting material, the sequence space for the first ligation stage creating 24mers can still be completely sampled. We obtained more than 12 million individual strands by Next Generation Sequencing (NGS), showing a significant selection of sequences undergoing this elongation dynamic. Utilizing a self-written LabView code we can study starting sequence pool biases, short- and long- range correlation in sequence space, base-fraction evolution as a function of product length as well as the driving selection mechanics as a function of product length. In addition, we show temperature, concentration and sequence-space dependent dynamics of this system by PAGE.