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BP: Fachverband Biologische Physik
BP 39: Cytoskeletal Filaments II
BP 39.1: Vortrag
Freitag, 20. März 2020, 09:30–09:45, SCH A251
Mechanosensitive Self-Assembly of Myosin II Minifilaments — •Justin Grewe1, Kai Weißenbruch2, Martin Bastmeyer2, and Ulrich S. Schwarz1 — 1Ruprecht Karl University Heidelberg, Germany — 2Karlsruhe Institute for Technology, Germany
Self-assembly and force generation are two central processes in biological systems that usually are considered in separation. However, the signals that activate non-muscle myosin II molecular motors simultaneously lead to self-assembly into myosin II minifilaments as well as progression of the motor heads through the crossbridge cycle.
We investigate theoretically the possible effects of coupling these two processes. Our assembly model, which builds upon a consensus architecture of the minifilament, predicts a critical aggregation concentration at which the assembly kinetics slow down dramatically. The combined model predicts that increasing actin filament concentration and force both lead to a decrease in the critical aggregation concentration in addition to force decelerated myosin turnover. We benchmark our model, in particular the turnover, against in-vivo fluorescence recovery after photobleaching experiments we performed in different experimental conditions and find reasonable agreement with the model.
We suggest that due to these findings, myosin II minifilaments in a filamentous context couple self-assembly with force generation and by this effect might be in a critical state that reacts faster to varying conditions than in solution.