Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 4: Membranes and Vesicles (joint session BP/CPP)
BP 4.6: Vortrag
Montag, 16. März 2020, 11:30–11:45, ZEU 250
Prerequisites and kinetics of lipid bilayer fusion with living cell membrane — •Justus Bednár1,2, Anastasia Svetlova1,2, Vanessa Maybeck1, and Andreas Offenhäusser1 — 1Forschungszentrum Jülich, Institute of Complex Systems: Bioelectronic (ICS-8) — 2Fakultät für Mathematik, Informatik und Naturwissenschaften RWTH Aachen
Fusion processes between artificial lipid vesicles and living cell membrane are studied for a variety of reasons. The delivery of anti-cancer therapeutics or the method known as lipofection are only two applications that would benefit from a detailed understanding of the prerequisites and kinetics of this fusion process.
While usually this process takes place between liposomes that have a small size relative to the cell membrane they are fusing to, an inverse approach is presented in the current work. Producing an artificial solid-supported lipid bilayer (SLB) first and letting extracts of living cell membrane fuse with it afterward allows for the application of a quartz crystal microbalance with dissipation monitoring (QCM-D). Tracking the changes in resonance frequency and energy dissipation of a quartz sensor underneath the SLB allows for real-time tracking of adhesion and fusion processes.
Using the proposed setup along with dynamic light scattering and fluorescence microscopy, the dependence of fusion efficiency and kinetics on lipid composition of the artificial lipid bilayer as well as on the concentration of cell membrane vesicles is evaluated.