Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 4: Membranes and Vesicles (joint session BP/CPP)
BP 4.7: Vortrag
Montag, 16. März 2020, 11:45–12:00, ZEU 250
Highly Reproducible Physiological Asymmetric Membrane with Freely Diffusing Embedded Proteins in a 3D Printed Microfluidic Setup — Paul Heo1, Sathish Ramakrishnan1,2, Jeff Coleman2, James E. Rothman2, •Jean Baptiste Fleury3, and Frederic Pincet1 — 1Laboratoire de Physiqe Statistique ENS, Paris, France — 2Department of Cell Biology Yale School of Medicine, New Haven, USA — 3Department of Experimental Physics and Center for Biophysics, Saarland University Saarbruecken, Germany
Experimental setups to produce and to monitor model membranes have been successfully used for decades and brought invaluable insights into many areas of biology. However, they all have limitations that prevent the full in vitro mimicking and monitoring of most biological processes. Here, a suspended physiological bilayer-forming chip is designed from 3D-printing techniques. This chip can be simultaneously integrated to a confocal microscope and a path-clamp amplifier. The bilayer, formed by the zipping of two lipid leaflets, is free-standing, horizontal, stable, fluid, solvent-free, and flat with the 14 types of physiologically relevant lipids, and the bilayer formation process is highly reproducible. Because of the two channels, asymmetric bilayers can be formed by making the two lipid leaflets of different composition. Furthermore, proteins, such as transmembrane, peripheral, and pore-forming proteins, can be added to the bilayer in controlled orientation and keep their native mobility and activity. These features allow in vitro recapitulation of membrane process close to physiological conditions.
Small, 2019, 10.1002/smll.201900725