Dresden 2020 – scientific programme

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BP: Fachverband Biologische Physik

BP 5: Bioimaging and Biospectroscopy I

BP 5.4: Talk

Monday, March 16, 2020, 16:00–16:15, HÜL 386

Stimulated Raman scattering microscopy of biomedical systems — •Moritz Floess, Florian Werner, Tobias Steinle, and Harald Giessen — 4th Physics Institute and Research Center SCoPE, University of Stuttgart, Germany

We employ stimulated Raman scattering (SRS) microscopy as a label-free and chemically selective imaging technique to investigate a system of pectin and porcine pleura. This model system is of high interest to improve surgical treatment of lung injuries. SRS is based on addressing molecular vibrational states using two pulsed laser beams with a variable frequency detuning. Hereby, an 8-W, 1032-nm, 450-fs Yb:KGW oscillator with 41 MHz repetition rate serves as the Stokes beam and simultaneously as the pump source for an optical parametric oscillator (OPO). The frequency-doubled OPO output provides the tunable Raman pump beam. Thanks to the favorable noise properties of the solid-state laser system we can address distinctive Raman bands in the fingerprint region with a high signal-to-noise ratio.