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BP: Fachverband Biologische Physik
BP 8: Poster I
BP 8.14: Poster
Montag, 16. März 2020, 17:30–19:30, P2/1OG
A theoretical framework to describe influence of electric field on Mesechymal cell differentiation — •Jonathan Dawson1, Ursula van Rienen1,2,3, and Revathi Appali1,3 — 1Institute of General Electrical Engineering, University of Rostock, Albert-Einstein-Str.2, 18059, Rostock — 2Life, Light and Matter, Interdisciplinary Faculty, University of Rostock — 3Ageing of Individuals and Society, Interdisciplinary Faculty, University of Rostock
Bone regeneration is a highly complex and tightly regulated process. Concerted and controlled action of human mesenchymal stem cell (hMSC) proliferation and differentiation into osteoblasts is pivotal in bone regeneration. Multiple biochemical and physiological factors influence the osteogenic differentiation and proliferation of hMSCs. Electromagnetic field (EMF) stimulation has been successfully used for the treatment of bone disorders. However, it is still unclear how exactly EMF influences the MSC dynamics. In close collaboration with experiments, we developed a theoretical framework to understand the effect of externally applied electric fields on hMSCs. In experiments, hMSCs were cultured in a chamber exposed to low-frequency electrical field applied via a transformer-like-coupling (TLC) [Hess et al. (2012)]. Cell differentiation was measured by cell alkaline phosphate (ALP) activity. Our mean-field theory describes the dynamics of a population of ALP stained hMSCs and takes into account cell division, cell apoptosis, cell differentiation, and intracellular ALP activity. Our model can account for the differences in the experimentally observed time course behaviour of total number of cells and the total ALP activity.