Dresden 2020 – scientific programme
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BP: Fachverband Biologische Physik
BP 8: Poster I
BP 8.17: Poster
Monday, March 16, 2020, 17:30–19:30, P2/1OG
A multidisciplinary approach to defining the identity and dynamics of adult gastric isthmus stem cells — •Seungmin Han1, 2, Juergen Fink2, Jong Kyoung Kim3, Benjamin Simons1, 2, and Bon-Kyoung Koo4 — 1WT-CRUK Gurdon Institute, Cambridge, UK — 2WT-MRC Cambridge Stem Cell Institute, Cambridge, UK — 3DGIST, Daegu, Republic of Korea — 4Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna, Austria
The gastric corpus epithelium is the thickest part of the gastro-intestinal tract and is characterized by rapid tissue turnover. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still usnder debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with actively-cycling stem cells maintaining the pit-isthmus-neck region and slow-cycling reserve stem cells maintaining the base. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly-cycling IsthSCs maintain the pit-isthmus-neck of corpus glands. Finally, single-cell RNA-seq analysis is used to define the molecular identity and lineage relationship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs.