Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Poster I
BP 8.20: Poster
Montag, 16. März 2020, 17:30–19:30, P2/1OG
The morpho-rheological phenotype of hematopoietic stem cells as a novel marker for transplantation — •Angela Jacobi1,2,3, Ahmad A Nawaz1,2, Martin Kräter1,2, Martin Bornhäuser3, and Jochen Guck1,2 — 1MPL, Erlangen, Germany — 2BIOTEC, TU Dresden, Dresden, Germany — 3University Hospital Carl Gustav Carus, TU Dresden, Dresden, Germany
Hematopoietic stem cells (HSCs) are transplanted after chemotherapy to reconstitute all blood cells. Unfortunately, analysis of their surface protein expression by standard flow cytometry does not yield functional information. Morpho-rheological properties of HSCs, determined predominantly by the cells cytoskeleton, play an important role for their function and can serve as a label-free marker for their identification. Numerous methods to measure morpho-rheological properties of cells are currently available, but most of them are limited in the ability to screen large heterogeneous populations in a robust and efficient manner, a feature required for successful translational applications. With real-time deformability cytometry (RT-DC) mechanical properties of cells in suspension can be screened continuously at rates of up to 1,000 cells/s, similar to conventional flow cytometers, which makes it a suitable method for HSC screening. Based on RT-DC measurements, we establish here a specific morpho-rheological fingerprint of HSCs that allows to distinguish them from all other blood cell types. We further show that this morpho-rheological phenotype allows for sorting of HSCs from heterogeneous human bone marrow samples, which could find practical application in HSC transplantation.