Dresden 2020 – scientific programme

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BP: Fachverband Biologische Physik

BP 8: Poster I

BP 8.2: Poster

Monday, March 16, 2020, 17:30–19:30, P2/1OG

Dynamics of pressure-induced phase transitions in concentrated lysozyme solutions — •Marc Moron¹, Clementine Lovato², Ahmed Al-Masoodi², Lisa Randolph², Mario Reiser^2,3, Johannes Möller³, Fabian Westermeier⁴, Göran Surmeier¹, Jennifer Bolle¹, Michael Paulus¹, Metin Tolan¹, and Christian Gutt² — ¹Fakultät Physik / DELTA, TU Dortmund, 44221 Dortmund, Germany — ²Department Physik, Universität Siegen, 57072 Siegen, Germany — ³European X-ray Free Electron Laser Facility, Holzkoppel 4, Schenefeld, Germany — ⁴Deutsches Elektronen Synchrotron DESY, D-22607 Hamburg, Germany

Phase transitions in concentrated protein solutions have been in the focus of research for years. For example, many diseases can be attributed to protein aggregation or liquid-liquid phase separation in human cells. Such systems can be modeled as crowded protein solutions. Lysozyme represents a well-studied model protein. We investigated the effect of hydrostatic pressure on concentrated lysozyme solutions in different environments and were able to show that besides temperature, protein concentration, cosolvents and ionic strength also the hydrostatic pressure modulates the protein-protein interaction. However, only the static properties of the lysozyme solutions were characterized. In this work, we present first pressure dependent X-ray photon correlation spectroscopy (XPCS) measurements on concentrated lysozyme solutions to study the dynamics of pressure-induced liquid-liquid phase transitions.