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BP: Fachverband Biologische Physik

BP 9: Poster II

BP 9.26: Poster

Montag, 16. März 2020, 17:30–19:30, P2/2OG

Ordering of n-alkanes in lipid bilayers — •Anika Wurl and Tiago Mendes Ferreira — Inst. f. Physik - NMR, Martin-Luther Univ. Halle-Wittenberg, Halle (Saale), Germany

In this work we probe the organisation of n-alkanes in different phospholipid bilayers by determination of C-H order parameters. Though short n-alkanes have been shown to incorporate into lipid membranes [1], the dependency of alkane solubility on chain-length and volume fraction has not been investigated in detail.

Order parameters for both alkanes and lipid tails were measured by 2H NMR and proton-detected local field NMR [2]. Results from both methods agreed and showed increased ordering of the lipid bilayers upon addition of n-alkanes. Experimental order parameters were compared to order parameters calculated from molecular dynamics simulations. Using the CHARMM36 forcefield [3], simulations captured experimentally observed trends and suggested a preferred accumulation of n-alkanes between bilayer leaflets. Our results further showed that at constant volume fraction, n-alkane solubility and ordering decreased with increasing chain length. In addition, alkane organisation within the lipid bilayer was strongly dependent on bilayer hydration and the melting temperatures of both alkane and lipid.

[1] J.M. Pope et al., Biochim. Biophys. Acta 1989, 980, 69 [2] X. Zhao et al., Chem. Phys. Lett. 2001, 342, 353 [3] J. B. Klauda et al., J. Phys. Chem. B 2010, 114, 7830.