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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 62: Single Molecule Biophysics (joint session BP/CPP)
CPP 62.6: Vortrag
Mittwoch, 18. März 2020, 10:45–11:00, SCH A251
How to gain reliable information from short trajectories — •Marie Schwebs1, Torsten Paul2, Marius Glogger1, Philip Kollmannsberger2, Markus Engstler1, and Susanne Fenz1 — 1Department for Cell and Developmental Biology, Biocenter, University of Würzburg, Germany — 2Center for Computational and Theoretical Biology, University of Würzburg, Germany
Trypanosoma brucei expresses a dense coat of GPI-anchored variant surface glycoproteins (VSGs). The fluidity of this coat is fundamental for the evasion of the host`s immune system and thus for the survival of the parasite. So far, the VSG dynamics on living trypanosomes has been studied at the micron and second scale for the whole ensemble. In this project, we want to elucidate the dynamics of individual VSGs in relation to the flagellar pocket, the sole site for endo- and exocytosis, with single-molecule fluorescence microscopy. For this purpose, we have recently introduced super-resolution imaging of intrinsically fast-moving flagellates based on cyto-compatible hydrogel embedding. Building on this work, we are now able to track VSG dynamics on living trypanosomes at high spatial (localization precision ~30 nm) and temporal resolution (f = 100 Hz). The length of gained trajectories is mainly limited by the shape and size of trypanosomes (approx. 18 μm in length and 3 μm in width). Therefore, we use a self-written program based on an approach from Hoze and Holcman [Biophys. J., 2014] to make reliable statements about local forces and the diffusion tensor. The information is gained from a large number of short trajectories and will be presented in directed motion and diffusion maps.