Dresden 2020 – scientific programme

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 95: Focus: Polymers under confinement II

CPP 95.3: Talk

Thursday, March 19, 2020, 15:30–15:45, ZEU 222

Physics of driven DNA polymers in confinement — •Ulrich Keyser — Cavendish Laboratory, University of Cambridge, JJ Thomse Ave, Cambridge, CB3 0HE, UK

Nanopore sensing has emerged as one of the most promising means for the analysis of single molecules. For DNA sequencing as well as molecule identification the physics governing molecular shape, velocity and fluctuations have to be understood and controlled. Here we show that we can design DNA molecules to measure velocity fluctuations during individual single molecule events [1]. The measurements reveal that friction between molecule, fluid and nanopore plays a crucial role. Based on these results we use electro-osmotic flow to force DNA to go through nanopores unfolded - facilitating molecular sensing [2]. Finally we investigated the details of polymer fluctuations and find that velocity and correlations crucially depend on the configurations of the molecules before initiation of translocation [3]. Our results guide efforts in developing DNA storage systems using nanopores [4].

[1] N. A. W. Bell, et al. Asymmetric dynamics of DNA entering and exiting a strongly confining nanopore. Nature Communications, 8:380, 2017. [2] N. Ermann, et al. Promoting single-file DNA translocations through nanopores using electroosmotic flow. The Journal of Chemical Physics, 149:163311, 2018. [3] K. Chen, et al. In preparation. [4] K. Chen, et al. Digital Data Storage Using DNA Nanostructures and Solid-State Nanopores. Nano Letters, 19:1210, 2019.