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KFM: Fachverband Kristalline Festkörper und deren Mikrostruktur
KFM 15: Postersession KFM
KFM 15.7: Poster
Donnerstag, 19. März 2020, 16:00–18:30, P2/1OG
Wide-field real-time nonlinear optical microscope in living organism — •Niklas Bethke1,2, Dustin Dzikonski1,2, Laura Vittadello1,2, and Mirco Imlau1,2 — 1Department of Physics, Osnabrück University, Germany — 2Center for Cellular Nanoanalytics, Osnabrück University, Germany
Nonlinear optical microscopy have emerged as a successful tool within the bio-medical research field enabling the possibility to do imaging in intact-live organism. In particular this type of microscope have to enable (i) high peak intensity to exploit non-linear effect, (ii) a field of view in the millimeter regime to ensure a proper imaging of the organism and (iii) the possibility to do time resolved experiment in the millisecond regime, where the most interesting biological effect occur. To embeded all this requirement in a single setup, the combination of an appropriate marker and the development of a non-conventional microscope setup is required. In this respect a femtosecond laser is coupled to an confocal laser scanning microscope, enabling the unique possibility (i) to control the non-linear effect by means of repetition rate (single shot - 80 MHz) and pulse duration (30 fs - 10 ps); (ii) to image living organism with a field of view up to about 1 mm and (iii) acquire images with an exposure time in range of tens of millisecond. Furthermore, niobate nanoparticles, such as KNbO3, are examined as marker candidate. They are of increasing importance as multimodal nanophotonic probes in biological environments due to their biocompatibility and pronounced nonlinear optic (NLO) properties. Financial support (DFG INST 190/179 FUGB) is gratefully acknowledged.