Dresden 2020 – scientific programme

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KFM: Fachverband Kristalline Festkörper und deren Mikrostruktur

KFM 6: Diamond

KFM 6.5: Talk

Tuesday, March 17, 2020, 11:10–11:30, TOE 317

Immobilization of Proteins on Ultrananocrystalline Diamond Surfaces — •Daniel Merker¹, Daniela Bertinetti², Katrin Schröder³, Monika Stengl³, Friedrich Herberg², Johann Peter Reithmaier¹, and Cyril Popov¹ — ¹Institute of Nanostructure Technologies and Analytics, Universität Kassel, Deutschland — ²Department of Biochemistry, Universität Kassel, Deutschland — ³Department of Animal Physiology, Universität Kassel, Deutschland

To investigate the molecular mechanisms of the inner clock in the madeira cockroach the detection of coupling factors is required. These coupling factors are secreted neuropeptides and neurotransmitters necessary to synchronize clock neurons into ensembles which direct the circadian rhythm. In this work we study the possible application of UNCD as a substrate for antibody immobilization to detect these coupling factors. Initially, various covalent coupling routes were investigated to immobilize the green fluorescent protein (GFP). Besides coupling chemistry with functional groups introduced directly on the UNCD surface by plasma, photochemical grafting on H-terminated as-grown UNCD was also investigated. The covalent coupling did not work since the functional groups on the UNCD surface were not reactive enough. In contrast, the photochemical grafting showed successful immobilization of GFP on surfaces with patterned modification (H-terminated squares on O-terminated surface). Since the O-terminated UNCD films have been already successfully applied for long-term cultivation of clock neurons we aim to utilize UNCD as antibody platform and to realize a time-resolved detection of coupling factors.