Dresden 2020 – scientific programme

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O: Fachverband Oberflächenphysik

O 124: Development of Novel Methods II

O 124.5: Talk

Friday, March 20, 2020, 11:30–11:45, WIL C107

Electrospray Ion Beam Deposition as Universal Preparation Method for Electron Microscopy Imaging of Molecules on Ultrathin Supports — •Stephan Rauschenbach — Department of Chemistry, University of Oxford Mansfield Road, Oxford, UK

Recent developments in electron microscopy (EM) instrumentation, such as aberration corrected lenses and high speed, single electron detectors have enabled the observation of individual atoms and molecules at sub-angstrom resolution. This finds applications in structural biology through cryo-EM, however the imaging of many species is hindered by absence of a general sample preparation method.

Here we present soft-landing electrospray ion beam deposition (ES-IBD)as universal preparation method for single molecule imaging on ultra-thin substrates (graphene/carbon membranes) EM. We show applications ranging from native folded proteins to small, few-atom cluster species. The experiment enables structure determination based on direct imaging or averaging in the case of radiation sensitive molecules. In addition, the control of the ion beam offered in ES-IBD allows for chemical isolation of a target species and for the controlled activation in surface collision.