Dresden 2020 – scientific programme

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O: Fachverband Oberflächenphysik

O 52: Poster Session - New Methods: Experiments

O 52.10: Poster

Tuesday, March 17, 2020, 18:15–20:00, P2/1OG

Selective Preparation of Electron Micrsocopy grids via Native Electrospray Ion-Beam Deposition — •Sam Britton, Tim Esser, Paul Fremdling, Stephan Rauschenbach, and Dhiren De Silva — University of Oxford

Due to the close relationship of structure and function, structural determination of proteins is an enormously important area of biochemistry. Cryo-electron microscopy (EM) can generate highly resolved structures by averaging many low contrast, single particle images of proteins embedded in vitreous ice; however, TEM is typically unable to reach high resolution for heterogeneous samples. Here we outline an alternative approach of preparing samples for single-particle EM, which is based upon native electrospray ion-beam deposition (ES-IBD). Samples are prepared using a modified commercial mass spectrometer (Q Exactive UHMR - Thermo Fisher) and subsequently imaged with atomic force microscopy and TEM. We explore the ability of this instrument to mass select single species followed by focused, soft deposition onto a TEM grid, thereby eliminating the issue of sample heterogeneity.