BPCPPDYSOE21 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Poster A: Single Molecule, Multicellular, Bioimaging, Focus Sessions, etc.
BP 11.17: Poster
Montag, 22. März 2021, 16:30–19:00, BPp
Resolving Energy Storage in Extra-Embryonic Membranes — •Zoë Lange1,2, Franziska Krämer1,3,4, Frederic Strobl3,4, Ernst Stelzer3,4, and Franziska Matthäus1,4 — 1Frankfurt Institute for Advanced Studies (FIAS) — 2IfB, FB Physik, Uni Frankfurt/Main — 3Buchmann Institute for Molecular Life Sciences (BMLS) — 4IZN, FB Biowissenschaften, Uni Frankfurt/Main
Efficient energy use and storage is crucial in living organisms. In the context of evolution, energy management is continuously optimized to ensure an individual’s ability to successfully compete. This is especially true for oviparous species, as all required energy has to be provided at the moment of oviposition in order to give rise to a fully functional organism. Based on our preliminary imaging data in the emerging insect model Tribolium castaneum, we formulate the hypothesis that extra-embryonic serosa cells utilize shape change during gastrulation to allocate and store energy that is later on required for their extensive movement during dorsal closure. To investigate this possible functional connection, we want to gain further insights into the multi-scale effects of force propagation from cellular to tissue level. Spatial and temporal dynamics of forces are calculated using non-invasive Force Inference (FI). FI utilizes a biomechanical model, a mathematical inverse method and a Bayesian framework to estimate cell and tissue stress from segmented image data and for the whole system simultaneously. Here we highlight our workflow from obtaining 3D time-lapse light sheet-based fluorescent microscopy images of live Tribolium embryos to multi-scale estimation of tensions and pressures acting in the serosa membrane.