BPCPPDYSOE21 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 11: Poster A: Single Molecule, Multicellular, Bioimaging, Focus Sessions, etc.
BP 11.19: Poster
Montag, 22. März 2021, 16:30–19:00, BPp
Migration of Cytotoxic T Lymphocytes in Collagen Matrices — •Zeinab Sadjadi1, Heiko Rieger1, Markus Hoth2, Bin Qu2, and Renping Zhao2 — 1Department of Theoretical Physics and Center for Biophysics, Saarland University — 2Department of Biophysics, Center for Integrative Physiology and Molecular Medicine, School of Medicine, Saarland University
Cytotoxic T lymphocytes (CTLs) need to migrate to search for their target cells in complex biological microenvironments, a key component of which is extracellular matrix (ECM). The mechanisms underlying CTL*s navigation are not well understood so far. Here we use a collagen assay as a model for the ECM and analyze the migration trajectories of primary human CTLs in collagen matrices with different concentrations. We observe different migration patterns for individual T cells. Three different motility types can be distinguished: slow, fast and mixed motilities. Slow CTLs remain nearly stationary within the collagen matrix and show slightly anti-persistent motility, while the fast ones move quickly and persistent. We hypothesize that the slow mode describes CTLs creating channels through the collagen matrix by deforming and tearing apart collagen fibers, and that the fast motility mode describes CTLs moving within these channels. The dynamics of the mixed type consists of periods of slow and fast motions. The dynamics can be well described by a two-state persistent random walk model. We extract the parameters of the model by analyzing experimental data.