BPCPPDYSOE21 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Poster A: Single Molecule, Multicellular, Bioimaging, Focus Sessions, etc.
BP 11.22: Poster
Montag, 22. März 2021, 16:30–19:00, BPp
Nanoprobing of osteoblasts adhered to molecular landscapes of dendrimer and protein — Christian Völkner1, Issam Assi1, Willi Karberg1, •Regina Lange1, Martina Grüning2, Barbara Nebe2, Ingo Barke1, and Sylvia Speller1 — 1University of Rostock, Institute of Physics, Physics of Surfaces & Interfaces, 18059 Rostock — 2Rostock University Medical Center, Dept. of Cell Biology, 18057 Rostock
Molecular surface gradients can constitute electric field landscapes and serve to control local cell adhesion and migration. This may allow the discovery of routes to improve osseointegration of implants. Flat molecule aggregate landscapes of amine-terminated dendrimers (PAMAM, generation 1) or proteins (BSA) were prepared on glass by micro contact printing [1] to provide lateral electric field gradients through their less negative zeta potentials compared to the glass substrate.
The local as well as the mesoscopic responses of adhered osteoblasts (MG-63) were studied by means of Scanning Ion Conductance Microscopy (SICM) [2] and Fluorescence Microscopy, in situ.
A distinct spindle shape oriented parallel to the stripe pattern as well as a preferential adhesion of the cells on the glass site have been observed when the width of the stripes and the spacing is 6 or 20 µm.
To explain this effect, we suggest a retraction mechanism according to cathodic taxis, a subtype of galvanotaxis [3].
[1] Whitesides et al., Chem. Rev. 105, 1171 (2005)
[2] Korchev et al., Biophys. J. 73, 653 (1997)
[3] Djamgoz et al., J.of Cell Science 117, 1631 (2004)