BPCPPDYSOE21 – scientific programme
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BP: Fachverband Biologische Physik
BP 11: Poster A: Single Molecule, Multicellular, Bioimaging, Focus Sessions, etc.
BP 11.23: Poster
Monday, March 22, 2021, 16:30–19:00, BPp
Kinetics of light-switchable surface association of C. reinhardtii populations — •Rodrigo Catalan1, Alexandros Fragkopoulos1, Nicolas von Trott1, Simon Kelterborn2, Peter Hegemann2, and Oliver Bäumchen1,3 — 1Max Planck Institute for Dynamics and Self-Organization (MPIDS), Am Fassberg 17, 37077 Göttingen, Germany — 2Humboldt University of Berlin, Institute of Biology, 10115 Berlin, Germany. — 3University of Bayreuth, Experimental Physics V, 95440 Bayreuth, Germany
Bacterial and microalgal colonization on surfaces produce favorable and adverse effects in technological and medical settings. Therefore, the fundamental aspects of biofilm formation on solid substrates are actively studied. While bacteria have been the main focus of research to understand microbial surface colonization, analogous studies using archetypes in microalgae are thus far elusive. We exploit light-switchable flagellar adhesion of C. reinhardtii [Kreis et al., Nature Physics, 2018] to study the kinetics of adsorption and desorption of cell suspensions on glass using bright field microscopy and image analysis. We observe that both processes exhibit a lag response relative to the time at which blue- or red-light conditions are set and we model this feature using time-delayed Langmuir kinetics. We find that adsorption occurs significantly faster than desorption, with the delay to be an order of magnitude larger. Adsorption experiments of phototactically blind C. reinhardtii mutants show that phototaxis does not affect the kinetics of either process. Hence, our method can be used as an assay for characterizing surface colonization.