BPCPPDYSOE21 – scientific programme
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BP: Fachverband Biologische Physik
BP 11: Poster A: Single Molecule, Multicellular, Bioimaging, Focus Sessions, etc.
BP 11.24: Poster
Monday, March 22, 2021, 16:30–19:00, BPp
Unravelling the biomolecular origin of light-switchable adhesion of Chlamydomonas to surfaces — •Antoine Girot1, Rodrigo Catalán1, Alexandros Fragkopoulos1, Marzieh Karimi1, Simon Kelterborn2, Peter Hegemann2, Michael Hippler3, and Oliver Bäumchen1,4 — 1Max Planck Institute for Dynamics and Self-Organization (MPIDS), 37077 Göttingen, Germany — 2Institute of Biology, Humboldt University of Berlin, 10099 Berlin, Germany — 3Institute of Plant Biotechnology and Biology, University of Münster, 48143 Münster, Germany — 4Experimental Physics V, University of Bayreuth, 95440 Bayreuth, Germany
In this work, we focus on the adhesion of the biflagellated microalga Chlamydomonas reinhardtii. We discovered that this alga exhibits light-switchable adhesion, i.e. the flagella of the cells stick to surfaces under blue but not under red light. In order to unravel the biomolecular origin of this specific light-regulated behaviour, two different experimental approaches are carried out. First, we record the kinetics of the adsorption and desorption of a cell suspension to a surface in response to a light switch. Second, we employ in vivo micropipette force spectroscopy to measure the adhesion force of single cells. By applying these methods for different wild-type strains, we aim at identifying characteristic gene sequences associated to cells adhesion. To unravel the blue-light sensitive photoreceptor responsible for adhesion, these experiments are performed with specific photoreceptor-deleted mutants. Finally, we investigate how the glycosylation of the flagellar membrane proteins affects the adhesion of Chlamydomonas.