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BP: Fachverband Biologische Physik

BP 11: Poster A: Single Molecule, Multicellular, Bioimaging, Focus Sessions, etc.

BP 11.30: Poster

Montag, 22. März 2021, 16:30–19:00, BPp

Asymmetries & gradients during early C. elegans embryogenesis — •Rebecca Benelli, Philipp Struntz, Dirk Hoffmann, and Matthias Weiss — Universität Bayreuth

To enable differentiation of cells and to facilitate cell organization the establishment of gradients is crucial in early embryogenesis. We have used the model organism C. elegans and a custom built light-sheet microscope to study the formation of protein and organelle gradients in three dimensions over time. Due to the low phototoxicity and reduced bleaching induced by this selective illumination long term observations without developmental perturbations are made possible. The focus of the current study is on evolution until the first cell division, which, next to the different sized daugther cells, is characterized by a lot of accompanying asymmetries. We study the protein concentration of two vital proteins in early development with respect to their axial as well as radial distribution. Also, two organelles with opposing gradients are investigated. Since diffusion plays a vital role in the establishment of gradients a new multiplexed diffusion measurement technique (SPIM-FCS) is used to quantify changes in diffusive behavior of proteins in space and time.