BPCPPDYSOE21 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 11: Poster A: Single Molecule, Multicellular, Bioimaging, Focus Sessions, etc.
BP 11.31: Poster
Montag, 22. März 2021, 16:30–19:00, BPp
Characterisation of local membrane height fluctuations on live cells — •Max Ulbrich1, Christian Völkner1, Regina Lange1, Sophie Kussauer2, Robert David2, Martina Grüning3, Barbara Nebe3, Ingo Barke1, and Sylvia Speller1 — 1Institute of Physics, Physics of Surfaces & Interfaces, University of Rostock, 18059 Rostock — 2University Medical Center, Cardial Regeneration, University of Rostock, 18057 Rostock — 3Rostock University Medical Center, 18057 Rostock
Assessment of cellular membrane fluctuations may aid monitoring of physiologic and pharmacologic effects [1]. Scanning Ion Conductance Microscopy (SICM) is a nanoprobing method to acquire morphology and dynamics on live cells. We operate the nanopipette-probe on fixed lateral locations and record SICM time traces in order to assess membrane fluctuations and cell activities [2]. Membrane fluctuations of live osteoblasts and cardiomyocytes are analysed in the time and frequency domain. Living osteoblasts and paused pacemaker cells, in average, exhibit scaling exponents of -2.8 and -2.5, respectively, however with large variations from cell to cell and site to site. We discuss this behavior in view of reference measurements on fixed cells and in the context of optically obtained results [3].
[1] B Rappaz, et al, Blood Cells Mol. Dis. 42 (2009) 228
[2] S-O Kim, et al, Nano Convergence (2017) 4:5
[3] B Sinha, et al, Biophys. J. (2017) 113