BPCPPDYSOE21 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Poster A: Single Molecule, Multicellular, Bioimaging, Focus Sessions, etc.
BP 11.40: Poster
Montag, 22. März 2021, 16:30–19:00, BPp
Transition of adherent to suspension state: relevance to cell mechanical properties — •Venkata Dabbiru1, Emmanual Manu1, Huy Tung Dau1, Nora Bödecker1, Doreen Biedenweg2, Ricardo Pires1, and Oliver Otto1 — 1University of Greifswald, Germany — 2University Medicine Greifswald, Germany
Adherent cells often detach from their native surface as a result of important physiological changes such as those, for example, found in cancer. While many studies have examined the mechanical properties of cells in their native adherent or suspended state, few studies have addressed the consequences associated with the transition between them. We have approached this question by using atomic force microscopy for adherent and semi-adherent cells as well as real-time deformability cytometry to study the mechanical properties of cells in suspension. As a model system, HEK293T cells have been cultured in the presence and absence of surface-tethering molecules, respectively, to mimic the transition state. Our results show that cell detachment is associated with increased stiffening of cells. Interestingly, surface-tethered transiently suspended cells and fully suspended cells differ in their mechanical properties. Analysing the F-actin distribution by confocal microscopy indicates a passive cell-surface interaction, which is not driven by adhesion molecules.