BPCPPDYSOE21 – scientific programme
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BP: Fachverband Biologische Physik
BP 23: Focus Biological Cells in Microfluidics II
BP 23.1: Talk
Tuesday, March 23, 2021, 14:00–14:20, BPc
ROS induces intracellular acidosis associated with increased cell stiffening — •Yesaswini Komaragiri1,3, Huy T Dau1, Doreen Biedenweg2, Ricardo H Pires1,3, and Oliver Otto1,3 — 1Biomechanics, ZIK-HIKE, Universität Greifswald, Greifswald, Germany — 2Universitätsmedizin Greifswald, Greifswald, Germany — 3Deutsches Zentrum für Herz-Kreislauf-Forschung e.V., Standort Greifswald, Universitätsmedizin Greifswald, Greifswald, Germany
Reactive oxygen species (ROS) are associated with important alterations in cell physiology. The impact that superoxides and other ROS have on the cytoskeleton has been extensively documented; however, the mechanism by which they may affect cell mechanics remain to be understood. By varying concentrations of hydrogen peroxide, we exposed the human myeloid precursor cell line (HL60) to different levels of ROS. Using real-time fluorescence and deformability cytometry, we coupled the mechanical characterization of cells with a simultaneous fluorometric assessment of intracellular superoxide levels. Our work reveals a direct correlation between the elastic modulus of cells and levels of superoxide. We did not detect global changes in the F-actin and microtubule network but demonstrate that cell stiffening at elevated ROS levels is driven by intracellular acidosis.