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BP: Fachverband Biologische Physik

BP 24: Poster B: Active Biological Matter, Cell Mechanics, Systems Biology, Computational Biophysics, etc.

BP 24.17: Poster

Dienstag, 23. März 2021, 16:00–18:30, BPp

Time-resolved MIET measurements of blood platelet spreading and adhesion — •Anna Zelená and Sarah Köster — Institute for X-Ray Physics, Georg-August-University Göttingen, Germany

Human blood platelets are non-nucleated fragments of larger cells (megakaryocytes) and highly important for blood clotting. The hemostatic function of platelets is directly linked to their mechanics and cytoskeletal morphology. However, the exact mechanism of spreading and contraction remains elusive. In our study, we employ metal-induced energy transfer (MIET) imaging in time-resolved and static modes to investigate, in vitro, single blood platelets with nanometer resolution. Using static MIET, we are able to quantitatively determine three-dimensional height profiles of the basal platelet membrane above a rigid metal substrate. We observe areas, where the basal platelet membrane approaches the rigid metal substrate more closely than the rest of the membrane. This may be related to previously observed ”hot spots” of high traction forces. Time-resolved MIET experiments allow us to follow the temporal evolution of the membrane-to-surface distance during adhesion and spreading. Our experiments reveal distinct behaviors between the outermost rim and the central part of the platelets. Overall, the combination of static and time-resolved MIET provides insights into the platelet adhesion system and improves our understanding of blood clot formation. Additionally, our approach demonstrates the potential of MIET as a three-dimensional reconstruction method for thin membrane formations.