BPCPPDYSOE21 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 24: Poster B: Active Biological Matter, Cell Mechanics, Systems Biology, Computational Biophysics, etc.
BP 24.42: Poster
Dienstag, 23. März 2021, 16:00–18:30, BPp
DNA Replication:Accuracy and Speed of elongation — •Mamata Sahoo1, Arsha Nousad1, Priyaranjan Baral2, and Stefan Klumpp3 — 1Department of Physics, University of Kerala, Kariavattom Campus-6955881, India — 2Department of Physics, — 3Institute for the Dynamics of Complex Systems, University of Gottingen, Gottingen, Germany
Being a dual purpose enzyme, the DNA polymerase is responsible for elongation of the newly formed DNA strand as well as cleaving the erroneous growth in case of a misincorporation. Though this is an efficient mechanism, sometimes DNAP with misincorporated nucleotide may escape to the next site as well as a correctly incorporated nucleotide causing a replication error may get cleaved unnecessarily from the exonuclease site. An error in 10 9 correct nucleotides incorporation has been observed experimentally. Here we propose a theory based kinetic model of DNA replication and find out the exact results for the velocity of elongation as well as the accuracy of replication. Surprisingly it is observed that the velocity of elongation with erroneous stepping passes through a crossover showing exact opposite behaviors at above and below the crossover point. Moreover, we ask the question that how the erroneous stepping with other parameters of the model have to be set in order to have a control over the speed of elongation mechanism. Finally we argue that the theoretical analysis of our results provide a simple picture of the design of a more accurate replication system and follows up with the speed-accuracy linear trade-off rule.